Sialotranscriptome sequencing and analysis of Anopheles sinensis and comparison with Psorophora albipes sialotranscriptome (Diptera: Culicidae)

Ya-Jie Fang; Zhen-Tian Yan; Bin Chen

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Sialotranscriptome sequencing and analysis of Anopheles sinensis and comparison with Psorophora albipes sialotranscriptome (Diptera: Culicidae)

机译：中华按蚊的唾液转录组测序和分析，并与Psorophora albipes sialotranscriptome（双翅目：葫芦科）进行比较

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Most of adult female mosquitoes secrete saliva to facilitate blood sucking,digestion and nutrition,and mosquito-borne disease prevention.The knowledge of classification and characteristics of sialotranscriptome genes are still quite limited.Anopheles sinensis is a major malaria vector in China and southeast Asian countries.In this study,the An.sinensis sialotranscriptome was sequenced using Illumina sequencing technique with a total of 10 907 unigenes to be obtained and annotated in biological functions and pathways,and 10 470 unigenes were mapped to An.sinensis reference genome with 70.46％ of genes having 90％-100％ genome mapping through bioinformatics analysis.These mapped genes were classified into four categories:housekeeping (6632 genes),secreted (1177),protein-coding genes with function-unknown (2646) and transposable element (15).The housekeeping genes were divided into 27 classes,and the secreted genes were divided into 11 classes and 96 families.The classification,characteristics and evolution of these classes/families of secreted genes are further described and discussed.The comparison of the 1177 secreted genes in An.sinensis in the Anophelinae subfamily with 811 in Psorophora albipes in the Culicinae subfamily show that six classes/subclasses have the gene number more than twice and two classes (uniquely found in anophelines,and Orphan proteins of unique standing) are unique in the former compared with the latter,whereas four classes/subclasses are much expanded and uniquely found in the Aedes class and is unique in the later.The An.sinensis sialotranscriptome sequence data is the most complete in mosquitoes to date,and the analyses provide a comprehensive information frame for further research of mosquito sialotranscriptome.

机译：大多数成年雌性蚊子分泌唾液以促进吸血，消化和营养以及蚊媒疾病的预防。唾液酸转录组基因的分类和特征的知识仍然十分有限。中华按蚊是中国和东南亚国家的主要疟疾传播媒介。本研究采用Illumina测序技术对中华按蚊的唾液腺基因组进行了测序，共获得10 907个单基因，并在生物学功能和途径上作了注释，并将10 470个单基因定位于中华按蚊的参考基因组，占70.46％。通过生物信息学分析可将基因组图谱定位为90％-100％的基因。将这些图谱基因分为四类：管家（6632个基因），分泌型（1177），功能未知的蛋白质编码基因（2646）和转座因子（15）。管家基因分为27类，分泌基因分为11类和96个科。这些分泌基因类/家族的cs和进化将进一步描述和讨论。对按蚊亚科中An.sinensis中1177个分泌基因与Culicinae亚科中的Psorophora albipes中811种分泌基因的比较表明，该基因有六个亚类。与后者相比，前者具有两倍以上的独特性，而前者具有独特的独特性，而前者具有两个类别（独特地存在于按蚊中，并且具有独特地位的孤儿蛋白），而伊蚊类别中有四个类别/亚类别则得到了极大的扩展和独特性。迄今为止，中华蚊的唾液腺转录组序列数据是目前为止最完整的，其分析为进一步研究蚊子唾液腺转录组提供了一个全面的信息框架。

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《中国昆虫科学：英文版》 |2018年第3期|368-378|共11页
作者
Ya-Jie Fang; Zhen-Tian Yan; Bin Chen;
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作者单位

Chongqing Key Laboratory of Vector Insects;

Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;

Chongqing Key Laboratory of Vector Insects;

Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;

Chongqing Key Laboratory of Vector Insects;

Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
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正文语种 eng
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Sialotranscriptome sequencing and analysis of Anopheles sinensis and comparison with Psorophora albipes sialotranscriptome (Diptera: Culicidae)

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