Cytotoxicity and binding profiles of activated Cry1Ac and Cry2Ab to three insect cell lines

Jizhen Wei; Gemei Liang; Kongming Wu; Shaohua Gu; Yuyuan Guo; Xinzhi Ni; Xianchun Li

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Cytotoxicity and binding profiles of activated Cry1Ac and Cry2Ab to three insect cell lines

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While Cry1Ac has been known to bind with larval midgut proteins cadherin,APN (amino peptidase N),ALP (alkaline phosphatase) and ABCC2 (adenosine triphosphate-binding cassette transporter subfamily C2),little is known about the receptors of Cry2Ab.To provide a clue to the receptors of Cry2Ab,we tested the baseline cytotoxicity of activated Cry1Ac and Cry2Ab against the midgut and fat body cell lines of Helicoverpa zea and the ovary cell line ofSpodopterafrugiperda (SF9).As expected,the descending order of cytotoxicity of Cry1Ac against the three cell lines in terms of 50％ lethal concetration (LC50) was midgut (31.0μg/mL) ＞ fat body (59.0μg/mL) and SF9 cell (99.6 μg/mL).By contrast,the fat body cell line (LC50 =7.55μg/mL) was about twice more susceptible to Cry2Ab than the midgut cell line (16.0μg/mL),the susceptibility of which was not significantly greater than that of SF9 cells (27.0μg/mL).Further,ligand blot showed the binding differences between Cry1Ac and Cry2Ab in the three cell lines.These results indicated that the receptors of Cry2Ab were enriched in fat body cells and thus largely different from the receptors of Cry 1Ac,which were enriched in midgut cells.

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《中国昆虫科学：英文版》 |2018年第4期|655-666|共12页
作者
Jizhen Wei; Gemei Liang; Kongming Wu; Shaohua Gu; Yuyuan Guo; Xinzhi Ni; Xianchun Li;
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State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;

Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ, USA;

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;

USDA-ARS, Crop Genetics and Breeding Research Unit, Tifton, GA, USA;

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;

Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ, USA;

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Cytotoxicity and binding profiles of activated Cry1Ac and Cry2Ab to three insect cell lines

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