Advances in the use of the RNA interference technique in Hemiptera

Jie Li; Xiao-Ping Wang; Man-Qun Wang; Wei-Hua Ma; Hong-Xia Hua

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Advances in the use of the RNA interference technique in Hemiptera

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RNA interference(RNAi)suppresses the expression of target genes by posttranscriptional regulation.Because double-stranded RNA(dsRNA)mediated gene silencing is a conserved mechanism in many eukaryotes,RNAi has become a valuable tool for unveiling gene function in many model insects.Recent research has also shown that RNAi can also be effective in the downregulation of target genes in Hemiptera.In this review,we discuss the use of the RNAi technique in gene functional analysis in hemipterans,highlighting the methods of dsRNA uptake by these insects and discuss the knock-down efficiency of these techniques.Although the RNAi technique has disadvantages,our primary goal here is to determine whether it can be exploited further in the discovery of new gene functions,and as a pest control strategy,in some important Hemipteran pests.

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《中国昆虫科学：英文版》 |2013年第1期|31-39|共9页
作者
Jie Li; Xiao-Ping Wang; Man-Qun Wang; Wei-Hua Ma; Hong-Xia Hua;
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作者单位

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory,Huazhong Agricultural University, Wuhan, China;

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory,Huazhong Agricultural University, Wuhan, China;

Colleage of Plant Science and Technology,Huazhong Agricultural University, Wuhan, China;

Colleage of Plant Science and Technology,Huazhong Agricultural University, Wuhan, China;

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory,Huazhong Agricultural University, Wuhan, China;

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Advances in the use of the RNA interference technique in Hemiptera

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