Vitellogenin content in fat body and ovary homogenates of workers and queens of Melipona quadrifasciata anthidioides during vitellogenesis

Vagner Tadeu Paes de Oliveira; Bruno Berger; Carminda da Cruz-Landim; Zilá Luz PaulinoSim(o)es

首页> 外文期刊>中国昆虫科学：英文版 >Vitellogenin content in fat body and ovary homogenates of workers and queens of Melipona quadrifasciata anthidioides during vitellogenesis

【24h】

Vitellogenin content in fat body and ovary homogenates of workers and queens of Melipona quadrifasciata anthidioides during vitellogenesis

机译：卵黄成虫期间工蚁和皇后鱼的脂肪体和卵巢匀浆中的卵黄蛋白原含量

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Vitellogenin (Vg) is an egg yolk protein that is produced primarily in the fat body of most female insects.In the advanced social structure of eusocial honeybees,the presence of the queen inhibits egg maturation in the workers' ovaries.However in the stingless bee Melipona quadrifasciata,the workers always develop ovaries and lay a certain amount of eggs while provisioning the brood cells with larval food during what is known as the worker nurse phase.The present work is a comparative study of the presence of Vg in homogenates of the fat bodies and ovaries of the nurse workers,and the virgin and physogastric queens ofM.quadrifasciata.The presence of Vg was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting using Apis mellifera anti-egg antibody.Vg was not detected in the fat bodies or ovaries of the workers,but it was found in the ovaries of virgin and physogastric queens and in the fat body ofphysogastric queens.The results are discussed,taking into account the reproductive state of the individuals and the other possible roles of Vg,such as a storage protein for metoabolism of other organs.

机译：卵黄蛋白原（Vg）是一种蛋黄蛋白，主要在大多数雌性昆虫的脂肪体内产生。在正常社会蜜蜂的先进社会结构中，女王的存在抑制了工人卵巢中的卵成熟。斑潜蝇（Melipona quadrifasciata），工人总是发育卵巢，并产下一定数量的卵，同时在所谓的工人护士阶段为雏鸡的细胞提供幼虫食物。本工作是对脂肪匀浆中Vg存在的比较研究。护理人员的身体和卵巢，以及quadrifasciata的处女和胃部皇后。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳和Apis mellifera抗卵抗体进行的免疫印迹法测定了Vg的存在。工人的卵巢，但发现于处女皇室和胃胃皇后的卵巢以及胃胃皇后的脂肪体内。考虑到个体的生殖状态和Vg的其他可能作用，例如其他器官的新陈代谢的储存蛋白。

著录项

来源
《中国昆虫科学：英文版》 |2012年第2期|213-219|共7页
作者
Vagner Tadeu Paes de Oliveira; Bruno Berger; Carminda da Cruz-Landim; Zilá Luz PaulinoSim(o)es;
展开▼
作者单位

Departamento de Biologia, Instituto de Bioci(e)ncias de Rio Claro, UNESP-Univ. Bela Vista, Rio Claro, S(a)o Paulo;

Departamento de Biologia, Instituto de Bioci(e)ncias de Rio Claro, UNESP-Univ. Bela Vista, Rio Claro, S(a)o Paulo;

Departamento de Biologia, Instituto de Bioci(e)ncias de Rio Claro, UNESP-Univ. Bela Vista, Rio Claro, S(a)o Paulo;

Departamento de Biologia, FFFL de Ribeir(a)o Preto, Universidade de S(a)o Paulo, USP, S(a)o Paulo, Brazil;

展开▼
收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 chi
中图分类
关键词

Vitellogenin content in fat body and ovary homogenates of workers and queens of Melipona quadrifasciata anthidioides during vitellogenesis

摘要

著录项

相关主题

期刊订阅