首页> 中文期刊> 《中国昆虫科学:英文版》 >Effects of starvation on the vitellogenesis, ovarian development and fecundity in the ectoparasitoid, Nasonia vitripennis (Hymenoptera: Pteromalidae)

Effects of starvation on the vitellogenesis, ovarian development and fecundity in the ectoparasitoid, Nasonia vitripennis (Hymenoptera: Pteromalidae)

         

摘要

A female-specific protein, vitellogenin (Vg), and its corresponding egg vitellin (Vt) are identified in the ectoparasific wasp Nasonia vitripennis. Both native Vt and Vg have a molecular mass of about 350 kDa, which is composed of two subunits of approximately 190 kDa and 165 kDa under reducing and denaturing conditions (sodium dodecyl sulfatepolyacrylamide gel electrophoresis). An indirect sandwich enzyme-linked immunosorbent assay developed with both monoclonal and polyclonal antibodies against N. vitripennis Vt.Vg was first detected in the hemolymph on the 10th day after parasitism, and was first observed in oocytes on the 12th day. In adults deprived of food, the highest hemolymph Vg level occurred at the time of adult eclosion and the highest level of Vt in ovaries was found at 30 h after eclosion. In contrast, feeding adults with 20% sucrose resulted in the reduction of Vt uptake by ovaries and the extension of life span, but had little effect on Vg production.Deprived of hosts, starvation of female wasps had no significant effects on ovariole growth and oocyte maturation before the wasps died. However, starvation of female wasps supplied with hosts accelerated the wasps laying progeny into hosts, but resulted in a decrease of total progeny production by comparison with wasps fed with 20% sucrose.

著录项

  • 来源
    《中国昆虫科学:英文版》 |2008年第5期|429-440|共12页
  • 作者单位

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    College of Life Sciences, China Jiliang University Hangzhou Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    College of Life Sciences, China Jiliang University Hangzhou Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

    State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, Jiangxi Province, China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 昆虫学;
  • 关键词

    Nasonia vitripennis; oocyte; starvation; vitellin; vitellogenin;

    机译:拟南芥(Nasonia vitripennis);卵母细胞;饥饿;卵黄蛋白;卵黄原蛋白;
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