聚丙烯凝胶电泳PAGE和毛细管电泳分析结果表明,蝶蛹金小蜂雌蜂个体和卵巢中明显存在雌性特异蛋白,即卵黄蛋白.但茧蜂科的螟长距茧蜂Macrocentrus linears和菜蛾盘绒茧蜂Cotesiaplutella雌雄虫体可溶性蛋白间无明显差异.SDS-PAGE梯度电泳结果表明,蝶蛹金小蜂卵黄原蛋白(Vg)和卵黄磷蛋白(Vt)均由二个分子量接近的亚基组成,分子量各为74.4和52.8KD.双向免疫扩散和PAGE梯度电泳都显示该蜂隐成蜂的雌性虫体及雌蜂血淋巴、脂肪体和卵巢中都有Vg或Vt,且卵黄原蛋白是由雌蜂脂肪体合成.%The results both from PAGE and capillary electrophoresis indicated that there was a female specific protein i.e. vitellogenin (Vg) or vitellin (Vt) in the female wasp of Pteromalus puparum (Hymenoptera: Pteromalidae). While there was no difference in the electrophoresis graph between soluble proteins of the female whole body and those of the male one both in two bracoids (Hymenoptera: Braconidae), i.e. Cotesia plutellae and Macrocentrus linears. According to the graph of the gradient SDS-PAGE, it was clear that the Vg or Vt of P. puparum consisted of two subunits with approximate molecular weights, and their molecular weights were 74.4 and 52.8 KDa, respectively. Both immunological reactions between some main different tissues of the female wasps and the male whole body and the polyantibody against the Vt of this parasitoid, and the graph of the gradient SDS-PAGE including soluble proteins sampled separately from hemolymph, fat body and ovary of the female and the whole body of the male demonstrated that Vg existed both in female fat body and hemolymph, and Vt deposited in the ovary, not in the male, as well as the Vg was synthesized in the female fat body.
展开▼
