A 6.12 kb XbaI-H fragment of the Helicoverpa armigera single nucleopolyhedrovirus (HaSNPV) gemone was cloned and the complete sequence of this fragment was sequenced by random sequencing method. Sequence comparison and analysis revealed an ORF13 which was homologous to ie-1 of Autographa californica nucleopolyhedrovirus (AcMNPV). The homologous encoding gene is ie-1. The total length of the encoding region of HaSNPV gene was 1986 bp and was predicted to encode 661 amino acid protein(IE-1 ) with molecular weight of 76.5 kD. The alingment of putative HaSNPV IE-1 amino acid sequence with those of other 9 reported baculoviruses IE-1s showed that the HaSNPV IE-1 was most closely related to Helicoverpa zea nucleopolyhedrovirus (HzNPV) IE-1, with 97% amino acid identidy. But it showed a low degree of sequence similarity to those of AcMNPV, Bombyx mori nucleopolyhedrovirus (BmNPV), Choristoneura fumiferana nucleopolyhedrovirus ( CfMNPV), Lymantria dispar nucleopolyhedrovirus(LdMNPV), Orgyia pseudotsugata nucleopolyhedrovirus(OpMNPV), Spodoptera exigua nucleopolyhedrovirus (SeMNPV), Plutella xylostella granulovirus(PxGV) and Xestia c-nigrum granulovirus (XcGV), with 23 %, 23 %,23%, 25%, 23 %, 14% , 27% and 7% amino acid identity, respectively. A phylogenetic tree of ten baculoviruses IE-1 was also given.%克隆了棉铃虫Helicoverpa armigera单粒包埋型核型多角体病毒(HaSNPV)C1株基因组DNA,并通过随机测序的方法测定了经XbaI酶切后的H片段的核苷酸全序列.序列比较和分析发现该片段中ORF13与苜蓿丫纹夜蛾Autographa californica多粒包埋型核型多角体病毒(AcMNPV)基因组ORF147(ie-1)同源.ie-1基因编码区全长1986bp,根据推测的氨基酸序列,可编码661个氨基酸残基组成的多肽,预计分子量为76.5kD.将所推导的HaSNPV IE-1氨基酸序列与其它已知的杆状病毒IE-1氨基酸序列进行比较,结果表明,HaSNPV和谷实夜蛾H.zea单粒包埋型核型多角体病毒IE-1氨基酸序列最为相似,同源性高达98%.与AcMNPV、家蚕Bombyx mori核型多角体病毒(BmNPV)、云杉卷叶蛾Choristoneura fumiferana多粒包埋型核型多角体病毒(CfMNPV)、舞毒蛾Lymantria dispar多粒包埋型核型多角体病毒(LdMNPV)、黄杉毒蛾Orgyia pseudotsugata多粒包埋型核型多角体病毒(OpMNPV)、甜菜夜蛾 Spodoptera exigua多粒包埋型核型多角体病毒(SeMNPV)、小菜蛾Plutella xylostella颗粒体病毒(PxGV)和Xestia c-nigrum颗粒体病毒(XcGV)的IE-1氨基酸序列同源性较低,分别为23%、23%、23%、25%、23%、14%、27%和7%.根据氨基酸序列由GENETYX程序绘制了已知杆状病毒IE-1的分子系统树.
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