CRISPR/Cas9-mediated ebony knockout results in puparium melanism in Spodoptera litura

Hong-Lun Bi; Jun Xu; Lin He; Yong Zhang; Kai Li; Yong-Ping Huang

首页> 中文期刊> 《昆虫科学：英文版》 >CRISPR/Cas9-mediated ebony knockout results in puparium melanism in Spodoptera litura

CRISPR/Cas9-mediated ebony knockout results in puparium melanism in Spodoptera litura

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Insect body pigmentation and coloration are critical to adaption to the environment.To explore the mechanisms that drive pigmentation,we used the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing system to target the ebony gene in the non-model insect Spodoptera litura.Ebony is crucial to melanin synthesis in insects.By directly injecting Cas9 messenger RNA and ebony-specific guide RNAs into S.litura embryos,we successfully induced a typical ebony-deficient phenotype of deep coloration of the puparium and induction of melanin formation during the pupal stage.Polymerase chain reaction-based genotype analysis demonstrated that various mutations had occurred at the sites targeted in ebony.Our study clearly demonstrates the function of ebony in the puparium coloration and also provides a potentially useful marker gene for functional studies in S.litura as well as other lepidopteran pests.

著录项

来源
《昆虫科学：英文版》 |2019年第6期|1011-1019|共9页
作者
Hong-Lun Bi; Jun Xu; Lin He; Yong Zhang; Kai Li; Yong-Ping Huang;
展开▼
作者单位

College of Life Sciences;

East China Normal University;

Shanghai;

China;

Key Laboratory of Insect Developmental and Evolutionary Biology;

Institute of Plant Physiology and Ecology;

Shanghai Institutes for Biological Sciences;

Chinese Academy of Sciences;

Shanghai;

China;

College of Life Sciences;

East China Normal University;

Shanghai;

China;

Department of Biology;

University of Nevada;

Reno;

NV;

USA;

College of Life Sciences;

East China Normal University;

Shanghai;

China;

Key Laboratory of Insect Developmental and Evolutionary Biology;

Institute of Plant Physiology and Ecology;

Shanghai Institutes for Biological Sciences;

Chinese Academy of Sciences;

Shanghai;

China;

展开▼
原文格式 PDF
正文语种 eng
中图分类
关键词

CRISPR/Cas9-mediated ebony knockout results in puparium melanism in Spodoptera litura

摘要

著录项

相关主题

期刊订阅