目的：研究转化生长因子β1(transforming growth factor -β1, TGF-β1)和热休克蛋白47(heat shock protein 47,HSP-47)在翼状胬肉和正常结膜组织表达的差异,从而探讨它们在翼状胬肉发生、发展中的作用,为翼状胬肉的治疗提供新的方向。 方法：采用免疫组化方法比较翼状胬肉和正常结膜组织中TGF-β1和HSP-47的表达差异。 结果：TGF-β1在翼状胬肉的鳞状上皮细胞中的表达水平明显高于正常结膜组织,在其基质层和内皮层有中等量表达,同时,在翼状胬肉纤维层的炎性细胞和血管内皮细胞中TGF-β1也有明显表达。与此相反,在正常结膜组织中TGF-β1表达较弱。 HSP-47在翼状胬肉的固有层高表达,在上皮细胞表达较弱,在正常结膜组织中则未见明显表达。 结论：相比正常结膜组织,TGF-β1和HSP-47均在翼状胬肉组织中有较高水平的表达,因此推测二者在诱导翼状胬肉的发生、发展中起着重要的作用。%AlM:To investigate the expression transforming growth factor-β1 (TGF-β1) and heat shock protein 47 (HSP-47), in pterygium and normal conjunctiva tissues, in order to study the roles of these cytokines played in the pathogenesis in human pterygium. METHODS:The expression difference of TGF-β1, HSP-47 between human pterygium and normal conjunctive tissues were compared by immuno - histochemistry technique. RESULTS:The positive expression of TGF-β1, HSP-47 was stronger than in normal conjunctive tissues ( P <0. 05), the TGF-β1 expressed in all layers of pterygium, especially in the squamous epithelium, in the inflammation cells and vascular endothelial cells also expressed. The HSP-47 showed higher expressed in the lamina propria layer of pterygium, and weakly expressed in epithelial layer, no obvious expression in normal conjunctive tissues. CONCLUSlON:Over-expression of TGF-β1 and HSP-47 in pterygium compared to the normal conjunctiva tissues may play a critical role during the occurrence, development and invasion of the pterygium.
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