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首页> 中文期刊> 《国际肝胆胰疾病杂志（英文版）》 >Immunosuppressive effects of rat mesenchymal stem cells:involvement of CD4+CD25+regulator y T cells

Immunosuppressive effects of rat mesenchymal stem cells:involvement of CD4+CD25+regulator y T cells

         
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摘要

BACKGROUND: Recent studies show that mesenchymal stem cells (MSCs) have immunomodulatory properties. They suppress the immune response to alloantigen and modify the proliferation of T cells. CD4+CD25+ regulatory T cells have strong immunomodulatory potential. However, little is known about the effects of rat MSCs (rMSCs) on the development of regulatory T cells. METHODS:MSCs were obtained from bone marrow of male Sprague-Dawley rats, and co-cultured with CD3+ T cells from allogeneic spleen cells. The proportion of CD4+CD25+ regulatory  T  cells  was  analyzed  by  lfow  cytometry.  To further conifrm the immunosuppressive activity of rMSCs, we used MTT assay and lfow cytometry of CD3+ T cells to investigate the proliferative responses of CD3+ T cells to mitogenic stimuli. Enzyme-linked immunosorbent assay was performed to detect alterations of the cytokines TNF-α, TGF-β and IL-10. RESULTS:The proliferation of CD3+ T cells decreased when co-cultured with rMSCs, and the degree of inhibition was concentration-dependent. The percentage of CD4+CD25+ regulatory T cells increased when CD3+ T cells were co-cultured with different concentrations of rMSCs. The levels of pro-inlfammatory cytokine (TNF-α) decreased while anti-inlfammatory (TGF-β, IL-10) cytokines increased in mixed lymphocyte reaction. CONCLUSIONS:rMSCs inhibit allogeneic T cell proliferation in mixed cell cultures. This immunosuppressive effect seems  to  be  mediated  by  inducing  the  generation  of CD4+CD25+ regulatory T cells and soluble factors.

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  • 来源
    《国际肝胆胰疾病杂志（英文版）》 |2008年第006期|608-614|共7页
  • 作者

    Zhou Ye; Yan Wang; Hai-Yang Xie and Shu-Sen Zheng;

  • 作者单位

    Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health;

    Key Laboratory of 0rgan Transplantation, Zhejiang Province;

    and Department of Hepatobiliary and Pancreatic Surgery, First Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Ye Z, Wang Y, Xie HY and Zheng SS;

    Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health;

    Key Laboratory of 0rgan Transplantation, Zhejiang Province;

    and Department of Hepatobiliary and Pancreatic Surgery, First Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Ye Z, Wang Y, Xie HY and Zheng SS;

    Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health;

    Key Laboratory of 0rgan Transplantation, Zhejiang Province;

    and Department of Hepatobiliary and Pancreatic Surgery, First Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Ye Z, Wang Y, Xie HY and Zheng SS;

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