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首页> 中文期刊> 《国际肝胆胰疾病杂志（英文版）》 >The methylation status of the TMS1/ASC gene in cholangiocarcinoma and its clinical signiifcance

The methylation status of the TMS1/ASC gene in cholangiocarcinoma and its clinical signiifcance

         
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摘要

BACKGROUND:TMS1/ASC is a bipartite protein comprising two protein-protein interactive domains:pyrin (PYD) and caspase recruitment domain (CARD). Proteins containing these domains play pivotal roles in regulating apoptosis and immune response pathways. The absence of TMS1/ASC expression in some tumors is because methylation of the TMS1/ASC gene contributes to carcinogenesis and cancer development. We studied the methylation status of the TMS1/ASC gene and its clinical signiifcance in cholangiocarcinoma. METHODS:Target DNA was modiifed by sodium bisulifte, coverting all unmethylated, but not methylated, cytosines to uracil, and subsequently by a nested ampliifcation with primers speciifc for methylated versus unmethylated DNA. The PCR product was detected by gel electrophoresis and combined with the clinical records of patients. RESULTS: Aberrant methylation of the TMS1/ASC gene was detected in specimens of colorectal cancer tissues from 13 (36.1%) of 36 patients, and specimens of adjacent normal tissues from 3 patients (8.3%). No statistical differences were seen in the extent of differentiation and invasion, lymph node metastasis, and pathologic type between the methylated and unmethylated tissues (P>0.05). CONCLUSIONS: The frequency of TMS1/ASC gene methylation in cholangiocarcinoma is high, but it is not related to pathologic changes. The TMS1/ASC gene is probably suppressed by methylation, and is resistant to apoptosis and immunological surveillance. The gene epigenetically affected in methylated tissues could be associated with carcinogenesis of cholangiocarcinoma.

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  • 来源
    《国际肝胆胰疾病杂志（英文版）》 |2006年第003期|449-453|共5页
  • 作者

    Xiao-Fang Liu; Shi-Guang Zhu; Hao Zhang; Zheng Xu; Hai-Long Su; Shao-Jun Li; Xian-Ting Zhou;

  • 作者单位

    Department of Hepatobiliary Surgery, Afifliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China Liu XF, Zhu SG, Xu Z, Su HL, Li SJ and Zhou XT, and Department of General Surgery, First Afifliated Hospital, China Medical University, Shenyang 110001, China Zhang H;

    Department of Hepatobiliary Surgery, Afifliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China Liu XF, Zhu SG, Xu Z, Su HL, Li SJ and Zhou XT, and Department of General Surgery, First Afifliated Hospital, China Medical University, Shenyang 110001, China Zhang H;

    Department of Hepatobiliary Surgery, Afifliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China Liu XF, Zhu SG, Xu Z, Su HL, Li SJ and Zhou XT, and Department of General Surgery, First Afifliated Hospital, China Medical University, Shenyang 110001, China Zhang H;

    Department of Hepatobiliary Surgery, Afifliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China Liu XF, Zhu SG, Xu Z, Su HL, Li SJ and Zhou XT, and Department of General Surgery, First Afifliated Hospital, China Medical University, Shenyang 110001, China Zhang H;

    Department of Hepatobiliary Surgery, Afifliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China Liu XF, Zhu SG, Xu Z, Su HL, Li SJ and Zhou XT, and Department of General Surgery, First Afifliated Hospital, China Medical University, Shenyang 110001, China Zhang H;

    Department of Hepatobiliary Surgery, Afifliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China Liu XF, Zhu SG, Xu Z, Su HL, Li SJ and Zhou XT, and Department of General Surgery, First Afifliated Hospital, China Medical University, Shenyang 110001, China Zhang H;

    Department of Hepatobiliary Surgery, Afifliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China Liu XF, Zhu SG, Xu Z, Su HL, Li SJ and Zhou XT, and Department of General Surgery, First Afifliated Hospital, China Medical University, Shenyang 110001, China Zhang H;

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