In vitro-derived insulin-producing cells modulate Th1 immune responses and induce IL-10 in streptozotocin-induced mouse model of pancreatic insulitis

Gholamreza Daryabor; Esmaeil Hashemi Shiri; Zahra Amirghofran; Eskandar Kamali-Sarvestani

首页> 中文期刊> 《国际肝胆胰疾病杂志（英文版）》 >In vitro-derived insulin-producing cells modulate Th1 immune responses and induce IL-10 in streptozotocin-induced mouse model of pancreatic insulitis

In vitro-derived insulin-producing cells modulate Th1 immune responses and induce IL-10 in streptozotocin-induced mouse model of pancreatic insulitis

开具论文收录证明 >>

期刊封面封底目录下载 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

cqvip:Background: Insulitis is defined by the presence of immune cells infiltrating in the pancreatic islets that might progress into the complete β-cell loss. The immunomodulatory properties of bone marrow-derived mesenchymal stem cells(BM-MSCs) have attracted much attention. This study aimed to evaluate the possible immunomodulatory effects of rat BM-MSCs and MSCs-derived insulin-producing cells(IPCs) in a mouse model of pancreatic insulitis. Methods: Insulitis was induced in BALB/c mice using five consecuti ve doses of streptozotocin. MSCs or IPCs were directly injected into the pancreas of mice and their effects on the expression of Th subsetsrelated genes were evaluated. Results: Both BM-MSCs and IPCs significantly reduced the expression of pancreatic Th1-related IFN-γ( P < 0.001 and P < 0.05, respectively) and T-bet genes(both P < 0.001). Moreover, the expression of IL-10 gene was significantly increased in IPC-treated compared to BM-MSC-or PBS-treated mice( P < 0.001 both comparisons). Conclusions: BM-MSCs and IPCs could successfully suppress pathologic Th1 immune responses in the mouse model of insulitis. However, the marked increase in IL-10 gene expression by IPCs compared to BM-MSCs suggests that their simultaneous use at the initial phase of autoimmune diabetes might be a better option to reduce inflammation but these results need to be verified by further experiments.

著录项

来源
《国际肝胆胰疾病杂志（英文版）》 |2021年第4期|376-382|共7页
作者
Gholamreza Daryabor; Esmaeil Hashemi Shiri; Zahra Amirghofran; Eskandar Kamali-Sarvestani;
展开▼
作者单位

Autoimmune Diseases Research Center Medical School Shiraz University of Medical Sciences Shiraz 71345-1798 Iran;

Autoimmune Diseases Research Center Medical School Shiraz University of Medical Sciences Shiraz 71345-1798 Iran;

Autoimmune Diseases Research Center Medical School Shiraz University of Medical Sciences Shiraz 71345-1798 Iran;

Department of Immunology Medical School Shiraz University of Medical Sciences Shiraz 71345-1798 Iran;

Autoimmune Diseases Research Center Medical School Shiraz University of Medical Sciences Shiraz 71345-1798 Iran;

Department of Immunology Medical School Shiraz University of Medical Sciences Shiraz 71345-1798 Iran;

展开▼
原文格式 PDF
正文语种 eng
中图分类
关键词

In vitro-derived insulin-producing cells modulate Th1 immune responses and induce IL-10 in streptozotocin-induced mouse model of pancreatic insulitis

摘要

著录项

相关主题

期刊订阅