目的 建立流式细胞术干/祖细胞分类计数方法,探讨其在自体造血干细胞移植治疗恶性血液系统疾病中的应用价值.方法 以抗CD45-PC5/CD34-PE/CD38-FITC建立三色流式细胞术造血干/祖细胞分类计数方法,对患者自体干细胞移植动员效果、单采物质量及回输单采细胞进行定量检测.结果 动员后样品检测分析发现,87.5%的患者动员效果较好,单个核细胞含量均在70.0%以上;CD34+细胞总数大于4×106/kg体质量者占90.0%.干细胞冻存样品检测分析发现,CD34+细胞数量冻存后存在一定衰减,但衰减量小于10.0%,经t检验证实冻存前后差异无统计学意义(P>0.05).结论 流式细胞术干/祖细胞分类计数在自体造血干细胞移植治疗恶性血液系统疾病中得到有效应用,单采细胞标本的定量检测为临床判断动员后单采时机、单采细胞质量、单采获得CD43+CD38-造血干细胞量、实际回输患者的CD43+CD38-造血干细胞量均提供了直接的科学数据.%Objective To establish a method of stem/progenitor cells differential count by flow cytometry to investigate the value in autologous hematopoietic stem cell transplantation for treatment of malignant diseases. Methods The differential count of hematopoietic stem/progenitor cell was established by a 3-color flow cytometry with Anti-CD45-PC5/CD34-PE/CD38-FITC and was used in autologous stem cell transplantation for treatment of malignant nosohemia. Results Mobilization of the sample test a-nalysis found that 87. 5% of patients mobilized better,mononuclear cell levels were above 70. 0% ;patients whose CD34 cell population were in the 4 × 106/kg body weight or more accounted for 90. 0%. Detection of stem cells in frozen samples found that CD34 + cells after cryopreservation certain decay, but less than 10. 0%, No significant difference was recorded between before and after cryopreservation by t test(PX). 05). Conclusion Stem/progenitor cells differential count by flow cytometry in autologous stem cell transplantation for the treatment of malignant blood diseases were effectively applied, single-cell samples collection for clinical quantitative analysis were used to provide a direct scientific data for judge the acquisition timing,cell quality,mass of CD43+CD38-hematopoietic stem cells in apheresis and actual retransfusion.
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