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《临床与病理杂志》
>Evaluating the clinical feasibility：the direct bisulfite genomic sequencing for examination of methylated status of protocadherin10 (PCDH10) promoter to predict the prognosis of gastric cancer
Evaluating the clinical feasibility：the direct bisulfite genomic sequencing for examination of methylated status of protocadherin10 (PCDH10) promoter to predict the prognosis of gastric cancer
Objective:To elucidate the clinical signiifcance of the methylated status of CpG site count of PCDH10 promoter in the survival prediction in gastric cancer (GC).Methods: In our previous study, we demonstrated that the methylated CpG site count was significantly associated with the survival of patients with gastric cancer using the bisulfite genomic sequencing (BGS) in the gastric cancer tissue with ifve clones per sample. It was so complicate for each patient underwent the BGS detection with clones.Results:PCDH10 promoter methylation was found 257 (54.6%) in all patients. GC patients with 5 or more methylated CpG site counts of PCDH10 promoter was significantly associated with poorer survival (P=0.039). On the multivariate survival analysis, we found that T stage, N stage and hypermethylated CpG site counts of PCDH10 DNA promoter were the independent predictors of prognosis for GC patients.Conclusion:Our present ifndings suggested that hypermethylated CpG site counts of PCDH10 DNA for evaluating the prognosis of gastric cancer was reasonable by using the direct sequencing.
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机译：国家集中采购试点政策对制药企业和制药产业的影响评估 =Evaluation of the Impact of National Centralized Procurement Pilot Policies on Pharmaceutical Companies and the Pharmaceutical Industry
