Molecular characterization of chalcone isomerase （CHI） regulating flower color in herbaceous peony （Paeonia lactiflora Pall.）

WU Yan-qing; ZHU Meng-yuan; JIANG Yu; ZHAO Da-qiu; TAO Jun

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Molecular characterization of chalcone isomerase （CHI） regulating flower color in herbaceous peony （Paeonia lactiflora Pall.）

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cqvip:Chalcone isomerase(CHI) is a key enzyme that converts yellow chalcone to colorless naringenin,playing an important regulatory role in color formation of ornamental flowers.We determined the coding sequence of CHI in herbaceous peony using rapid-amplification of c DNA ends(RACE) technology,and subsequently detected the expression pattern of CHI in the inner and outer petals at different developmental stages using q RT-PCR.We cloned the upstream promoter sequences of CHI using genome walking technology and predicted the location of Cp G islands and 5′ truncation.In addition,we constructed five dual-luciferase reporter gene carriers and detected the promoter activities of different fragments.Our results showed that the full-length c DNA sequence of CHI was 898 bp,and the 5′-upstream core promoter was located at –1 651 to –2 050 bp region,where contained one Cp G island(–1897 to –2 010 bp) and several important binding sites of transcription factor,such as Sp1,serum response factor(SRF),activating protein(AP)-2 alpha and CCAAT/enhancer binding protein(C/EBP)alpha.Expression results showed that the expression of CHI at different developmental stages was generally higher in inner petals than those in outer petals,and the maximum at the bud stage(S1).Thus,this study will provide theoretical basis for an in-depth study of CHI gene function and expression regulation.

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《农业科学学报：英文版》 |2018年第1期|P.122-129|共8页
作者
WU Yan-qing; ZHU Meng-yuan; JIANG Yu; ZHAO Da-qiu; TAO Jun;
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作者单位

Jiangsu Key Laboratory of Crop Genetics and Physiology;

College of Horticulture and Plant Protection;

Yangzhou University;

Yangzhou 225009;

P.R. China;

Jiangsu Key Laboratory of Crop Genetics and Physiology;

College of Horticulture and Plant Protection;

Yangzhou University;

Yangzhou 225009;

P.R. China;

Jiangsu Key Laboratory of Crop Genetics and Physiology;

College of Horticulture and Plant Protection;

Yangzhou University;

Yangzhou 225009;

P.R. China;

Jiangsu Key Laboratory of Crop Genetics and Physiology;

College of Horticulture and Plant Protection;

Yangzhou University;

Yangzhou 225009;

P.R. China;

Jiangsu Key Laboratory of Crop Genetics and Physiology;

College of Horticulture and Plant Protection;

Yangzhou University;

Yangzhou 225009;

P.R. China;

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Molecular characterization of chalcone isomerase （CHI） regulating flower color in herbaceous peony （Paeonia lactiflora Pall.）

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