Weaning methods affect ruminal methanogenic archaea composition and diversity in Holstein calves

DONG Li-feng; MA Jun-nan; TU Yan; DIAO Qi-yu

首页> 中文期刊> 《农业科学学报：英文版》 >Weaning methods affect ruminal methanogenic archaea composition and diversity in Holstein calves

Weaning methods affect ruminal methanogenic archaea composition and diversity in Holstein calves

开具论文收录证明 >>

期刊封面封底目录下载 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

The objective of the present study was to examine the effect of different weaning methods on the ruminal methanogenic archaea composition and diversity in Holstein calves.Thirty-six newborn Holstein bull calves were assigned to 1 of 3 treatments:(1)conventional weaning(d 56)and fed a high proportion of solid feed(CWS);(2)conventional weaning(d 56)and fed a high proportion of liquid feed(CWL);(3)early weaning(d 42)and fed with a high proportion of solid feed(EWS).High-throughput sequencing of the methyl coenzyme M reductase(mcr A)gene,which encodes theα-subunit of methyl coenzyme M reductase-the enzyme that catalyzes the final step in methanogenesis was used to determine the composition and diversity of rumen methanogens.No significant difference(P>0.05)was observed for operational taxonomic units(OTUs)or richness indices,but diversity indices increased(P<0.05)for calves fed high dietary solids.Predominant families across the three treatments were Methanobacteriaceae,Thermoplasmataceae and Methanomassiliicoccaceae.Calves in the EWS treatment had a higher(P<0.05)relative abundance of Methanobrevibacter sp.strain AbM4 and Methanosphaera stadtmanae,while calves in the CWL treatment had a higher(P<0.05)abundance of Methanosphaera sp.strain SM9.A positive(P<0.05)relationship was identified between butyrate and Methanobrevibacter sp.strain AbM4.In conclusion,the composition and diversity of methanogens in the rumen of Holstein calves varied under the different weaning methods.This study identified a positive relationship between butyrate and Methanobrevibacter sp.strain AbM4,potentially reflecting correlations between ruminal fermentation variables and methanogenesis function.These in-depth analyses provide further understanding of weaning methods for intensified production systems.

著录项

来源
《农业科学学报：英文版》 |2019年第5期|P.1080-1092|共13页
作者
DONG Li-feng; MA Jun-nan; TU Yan; DIAO Qi-yu;
展开▼
作者单位

[1]Feed Research Institute;

Chinese Academy of Agricultural Sciences/Beijing Key Laboratory for Dairy Cow Nutrition/Key Laboratory of Feed Biotechnology;

Ministry of Agriculture;

Beijing 100081;

P.R. China;

[1]Feed Research Institute;

Chinese Academy of Agricultural Sciences/Beijing Key Laboratory for Dairy Cow Nutrition/Key Laboratory of Feed Biotechnology;

Ministry of Agriculture;

Beijing 100081;

P.R. China;

[1]Feed Research Institute;

Chinese Academy of Agricultural Sciences/Beijing Key Laboratory for Dairy Cow Nutrition/Key Laboratory of Feed Biotechnology;

Ministry of Agriculture;

Beijing 100081;

P.R. China;

[1]Feed Research Institute;

Chinese Academy of Agricultural Sciences/Beijing Key Laboratory for Dairy Cow Nutrition/Key Laboratory of Feed Biotechnology;

Ministry of Agriculture;

Beijing 100081;

P.R. China;

展开▼
原文格式 PDF
正文语种 CHI
中图分类农业科学;
关键词
calf; methyl coenzyme M reductase(mcrA)gene; methanogenic archaea diversity; rumen fermentation; weaning methods;

机译：小牛;甲基辅酶M还原酶（mcrA）基因;产甲烷古菌多样性;瘤胃发酵;断奶方法;

Weaning methods affect ruminal methanogenic archaea composition and diversity in Holstein calves

摘要

著录项

相关主题

期刊订阅