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首页> 中文期刊> 《农学学报》 >低温胁迫对蓖麻种子萌发过程中生理生化影响

低温胁迫对蓖麻种子萌发过程中生理生化影响

         
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摘要

The purpose of the study is to explore the regulation mechanism of castor seed germination under low temperature,in order to select and breed new varieties with high quality and resistance.In this study,three castor cultivars were used as materials.A complete randomized design was adopted with 3 duplicates.The low temperature of 15℃ and normal temperature of 25℃ (CK) were set up and the seeds germination experiments were conducted.After determining the vitality of cultivars,the physiological index was determined by taking samples when the radicles were 2 cm long.The results indicated that seeds germination of castor was significantly hindered by low temperature stress.The sugar content and TSP were less than that of the control in radicles under low temperature,but the MDA content was significantly higher than that of the control under the low temperature.The performance of enzyme activity in castor was quite different under low temperature,SOD activity in radicles was less than that of the control under low temperature.Excepting ‘Tongbi No.6’,other varieties’ POD activity was significantly lower than that of the control;the CAT activity performance was not significant,and the activities were basically coincident under different temperature conditions.It concluded that ‘Tongbi No.5’ performed well under low temperature stress,and it could take gene sequencing for the next step.%旨在探索蓖麻种子在低温下萌发的调控机制,为选育优质抗逆蓖麻新品种做准备.笔者以3个蓖麻品种为材料,采用完全随机设计,重复3次,设置低温15℃、常温25℃(CK),进行种子萌发试验,确定品种活力后,待胚根生长至2 cm时,进行取样测定相关生理指标.结果表明:低温显著阻碍蓖麻种子萌发.低温胁迫下可溶性糖含量和可溶性蛋白含量显著低于对照,而丙二醛含量显著高于对照;低温胁迫下供试蓖麻品种各酶活性表现差异较大,胚根SOD活性均极显著低于对照;除‘通蓖6号’外,其余供试品种POD活性也极显著低于对照;CAT活性表现不显著,且不同温度条件下活性基本一致.通过试验得出‘通蓖5号’在低温胁迫下表现良好,可进行下一步基因测序.

著录项

  • 来源
    《农学学报》 |2017年第3期|5-8|共4页
  • 作者

    白雪; 李兴; 刘鹏; 郑根昌; 李旭新; 张春兰; 贾爱民;

  • 作者单位

    内蒙古民族大学农学院,内蒙古通辽028043;

    内蒙古民族大学农学院,内蒙古通辽028043;

    内蒙古民族大学农学院,内蒙古通辽028043;

    内蒙古自治区高校蓖麻产业工程技术中心,内蒙古通辽028043;

    内蒙古自治区蓖麻育种重点实验室,内蒙古通辽028043;

    内蒙古自治区蓖麻产业协同创新培育中心,内蒙古通辽028043;

    内蒙古民族大学农学院,内蒙古通辽028043;

    内蒙古民族大学农学院,内蒙古通辽028043;

    内蒙古民族大学生命科学学院,内蒙古通辽028043;

    内蒙古自治区国营免渡河农场,内蒙古牙克石022176;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 蓖麻;
  • 关键词

    蓖麻; 低温胁迫; 种子萌发; 生理生化;

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