[Objective] To analyze codon usage bias in Drosophila melanogaster and D.sechellia mitochondrion and NADH dehydrogenase subunit gene family.[Method] We calculated the related codon usage bias parameter using the software CodonW1.4.[Result] We found the AT content was higher than the CG content of the protein coding genes of the two kinds of fruit fly mitochondrion.The codon usage bias of D.sechellia mitochondrion was stronger than the codon usage of D.melanogastera.The codons whose RSCU was more than 1 end with "A or U" rather than end with "C or G" in the two kinds of fruit fly mitochondrion.The codon usage bias of NADH dehydrogenase subunit gene family of the two kinds of fruit fly mitochondrion may be affected by mutation pressure.D.melanogastera mitochondrion didn't contain the codon "CGC".The codon usage bias of NADH dehydrogenase subunit 3 gene was stronger than other NADH dehydrogenase subunit gene family members.Additionally,though NADH dehydrogenase subunit gene family prefers using the codons end with "A or U",the RSCU of codons AGA and GCA were both 0.[Conclusion] The study provided the new views for research of evolution of the two kinds of fruit fly mitochondrion.%[目的]分析2种果蝇(Drosophila melanogaster与D.sechellia)线粒体基因组及NADH dehydrogenase subunit基因的密码子偏好性.[方法]利用GodonW1.4计算密码子偏好性参数的相关参数.[结果]2种果蝇线粒体中蛋白编码基因的AT含量比GC含量更高.D.sechellia的线粒体基因组的密码子偏好性比黑腹果蝇D.melanogastera的线粒体基因组的密码子偏好性略强.2种果蝇线粒体基因组中各蛋白编码基因CDs中,RSCU值大于1的密码子都是以A或U结尾,而并不以C或G结尾.2种果蝇线粒体基因组及NADH dehydrogenase subunit基因簇基因的密码子偏好性都可能受到突变压力影响.D.melanogaster线粒体基因组中不存在密码子CGC.NADH dehydrogenase subunit3基因的密码子偏好性可能比其他NADH dehydrogenase subunit基因更高.此外,尽管大部分NADH dehydrogenase subunit基因偏爱使用A或U结尾的密码子,但是在NADH dehydrogenase subunit6基因中,有2个AU结尾的密码子(AGA、GCA)的RSCU也为0.[结论]该研究结果为2种果蝇线粒体的进化研究提供了新观点.
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