[目的]对从健康的新鲜西藏景天科植物圆齿红景天茎内分离得到的曲霉属菌株进行形态学观察以及从分子生物学水平进行分离鉴定.[方法]用载片培养法进行菌株的判定和生物学特性的试验,利用rRNA基因ITS间隔区建立进化树分析法,进行该真菌的系统发育阐明.[结果]菌落在改良型KB培养基上和PDA培养基上形态表现一致,起初菌落为白色绒毛状,边缘列片状,浓密而不透明,基部菌丝体厚、平坦,具沟纹、绒毛状质地,菌株为无性型.通过分子生物学的rRNA基因ITS间隔区系统发育建立进化树的方法鉴定分离出的菌株为曲霉属黄绿组真菌.[结论]通过对菌落外部形态的观察、分析以及从分子生物学角度的无性型rRNA基因ITS间隔区系统发育分析,得知分离得到的菌株属于半知菌亚门曲霉属黄绿组真菌的一种.%[Objective] The morphological observations of Aspergillus strains isolated from the stem of the healthy fresh Tibet crassulaceae plants were studied and the separation and identification were conducted from the molecular biological level.[Method] The determination of the strains and the biological characteristics of the strain were carried out with the method of carrying plate culture,and the evolution tree analysis was established using the rRNA gene ITS interval region,and the system development of the fungus was elucidated.[Result] Colony in modified form KB medium and form was consistent in PDA medium,at first colony for white villous,edge column flake,dense and opaque,mycelium thick at base fiat provides quality,grooving villous strains for asexual.The isolated strain of the evolutionary tree were identified as the yellow-green group fungus of Aspergillus by the molecular biology of the rRNA gene ITS interval regional system development.[Conclusion] Through the observation and analysis of the external morphology of the colony and the phylogenetic analysis of the ITS spacer region of the asexual rRNA gene from the point of view of molecular biology,it is known that the isolated strain belongs to the fungus of the yellow-green group of Aspergillus.
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