[目的]研究茄叶斑鸠菊的化学成分.[方法]利用硅胶色谱、大孔树脂层析、Sephadex LH-20和ODS反相层析等方法,对茄叶斑鸠菊提取物进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构.[结果]从茄叶斑鸠菊中分离得到了6个神经酰胺类化合物,分别鉴定为1-O-β-D-glucopyranosyl-(2S,3R,8E)-2-[(2'R)-2-hydroxypalmitoyl amino]-8-octadecene-1,3-diol、1-O-β-D-glucopyranosyl-(2S,3S,4R,8E)-2-[(2'R)-2-hydroxypalmitoyl amino]-8-octadecene-1,3,4-triol、Soya cerbroside I、(2S,3S,4R,8E)-2-[(2'R)-2-hydroxy tetracosanoyl amino]-8-octadecene-1,3,4-triol、(2S,3S,4R,8E)-2-[(2'R)-2-hydroxypalmitoyl amino]-8-octadecene-1,3,4-triol、(2S,3S,4R,8E)-2-[(2'R)-2-hydroxypentracosanoyl amino]-8-octadecene -1,3,4-triol.[结论]6个化合物均为首次从该植物中分离得到,为茄叶斑鸠菊活性成分的开发利用奠定基础.%[Objective] The research aimed to study the chemical components of Vernonia solanifolia.[Method] The components were isolated and purified by silica gel chromatography,macroporous resin chromatography,Sephadex LH-20 and ODS reverse phase chromatography and other methods,and the structure of the compound was identified by physicochemical properties and spectroscopic data.[Result] Six ceramide compounds were isolated from Vernonia solanifolia,and their structure were identified as 1-O-β-D-glucopyranosyl-(2S,3R,8E)-2-[(2'R)-2-hydroxypalmitoyl amino]-8-octadecene-1,3-diol;1-O-β-D-glucopyranosyl-(2S,3S,4R,8E)-2-[(2'R)-2-hydroxypalmitoyl amino]-8-octadecene-1,3,4-triol;Soyacerbroside Ⅰ;(2S,3S,4R,8E)-2-[(2'R)-2-hydroxy tetracosanoyl amino]-8-octadecene-1,3,4-triol;(2S,3S,4R,8E)-2-[(2'R)-2-hydroxypalmitoyl amino]-8-octadecene-1,3,4-triol;(2S,3S,4R,8E)-2 -[(2'R)-2-hydroxypentracosanoyl amino]-8-octadecene-1,3,4-triol.[Conclusion] Six compounds were isolated from Vernonia solanifolia for the first time,which laid the foundation for the development and utilization of the active ingredients of Vernonia solanifolia.
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