[目的]优化高活性纳豆激酶的纳豆生产工艺条件.[方法]从市售纳豆产品中分离筛选出高活性的菌株作为试验菌株,采用固态发酵的方法制备纳豆,对影响纳豆激酶产量的主要因素浸泡时间、接种量、发酵时间等进行考察,研究高活性纳豆激酶的工艺条件.[结果]利用对甲基苯磺酰L精氨酸甲酯(TAME)法测定纳豆激酶活性,确定最佳纳豆激酶酶活的纳豆生产工艺条件为浸泡时间8 h、接种量10%、发酵时间48 h.[结论]该研究可为高活性纳豆激酶的生产提供参考.%[Objective] To optimize the process conditions of high activity natto kinase.[Method]Natto was isolated from commercial natto products and used as the experimental strain.Natto was prepared by solid-state fermentation.The process conditions of high activity natto kinase were studied.The main factors affecting the yield of natto kinase were as follows:immersion time,inoculation amount and fermentation time.[Result]The activity of natto kinase was determined by TAME method.The optimum natto kinase production process conditions were determined as 8 h soaking time,10% inoculation amount,48 h fermentation time.[Conclusion] The study can provide reference for production of high activity natto kinase.
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