[目的]研究雅砻江长丝裂腹鱼胚胎形态发育及仔鱼生长.[方法]通过人工干法授精获得长丝裂腹鱼受精卵,在水温(15.9±0.6)℃下进行孵化,对其胚胎和仔鱼的发育过程进行了研究.[结果]长丝裂腹鱼成熟卵卵径为(2.89±0.30) mm,吸水充分膨胀后卵径为(4.17±0.09)mm.在水温(10.8±0.8)和(15.9±0.6)℃下胚胎均能孵化,(19.5±0.6)℃条件下胚胎发育至原肠中期即全部死亡.胚胎发育有效积温为1 275.46℃·h,生物学零度为6.27℃.在水温(15.9±0.6)℃的条件下,胚胎受精后3 h 35 min胚盘隆起,14 h45 min和34 h 50 min进入囊胚期和原肠胚期,51 h 35 min胚孔封闭,132 h 25 min开始出膜,217 h全部出膜.初孵仔鱼全长为(10.22±0.25)mm.全长(TL)与体重(BW)的关系式为:BW=1.209 3E-4(TL-10.54)2.512 8(R2 =0.975 7);体长(TL)和体重(BW)与日龄(D)的最佳关系式分别为:TL=0.380 5D3-0.002 6D2+2.868 7E-5D+ 10.741 8(R2=0.984 0),BW=1.010 82E-7(D +9.568 3)3.451 8(R2 =0.983 4).[结论]研究结果可为了解长丝裂腹鱼早期生活史、繁殖生物学等积累资料.%[Objective] To study the embryonic morphological development and larvae growth of Schizothorax dolichonema.[Method] The fertilized eggs of S.dolichonema were obtained by using dry fertilization technique and hatched at water temperature of (15.9 ± 0.6) ℃,and their embryonic and larval development were studied.[Result] The diameter of mature eggs of S.dolichonema was(2.89 ±0.30) mm and it was (4.17 ±0.09) mm after fully sopping up water.Embryos were successfully hatched at the water temperature of (10.8 ± 0.8) ℃ and (15.9 ± 0.6) ℃ respectively,but all embryos hatched at(19.5 ±0.6) ℃ died at the stage of mid archenteron.Effective accumulated temperature of S.dolichonema was 1 275.46 h · ℃,and its developmental biology zero was 6.27 ℃.At the water temperature of (15.9 ± 0.6) ℃,the blastodisc was formed 3 h and 35 min later,and then eggs entered blastocyst stage at 14 h and 45 min,and eggs entered gastrulation stage after 34 h and 50 min and blastopore closure stage after 51 h and 35 min.The first larvae hatched 132 h and 25 min after fertilization,and the last one hatched after 217 h.The total length of newly hatched larvae was (10.22 ± 0.25) mm.The regression equation between total length (TL),body weight (BW) and daily age (D) were BW =1.209 3E-4(TL-10.54)2.512 8 (R2 =0.975 7),TL =0.380 5D3-0.002 6D2 +2.868 7E-5D + 10.741 8(R2 =0.984 0),BW =1.010 82E-7 (D + 9.568 3) 3.451 8 (R2 =0.983 4),respectively.[Conclusion] The research results can accumulate materials for studying the early life history and reproductive biology.
展开▼
