[Objective] The aim was to study antibacterial activity of extract of Cycas siamensis seed.[Method] The antibacterial activity of extract of Cycas siamensis seed was studied by filter paper method,agar dilution method and fluorescent staining method.[Result] The extract had a broad-spectrum antibacterial activity,and the IC50 of Fusarium graminearum and spores were 1.65 and 2.93 mg/mL.The extracts changed hypha chitin and mitochondrial membrane potential.[Conclusion]The extract has stronger antibacterial activity.%[目的]研究铁树种子提取物的抑菌活性.[方法]采用纸片扩散法、牛津杯法、琼脂稀释法和荧光染色法,研究提取物抑菌活性.[结果]提取物具有光谱抗菌活性,对禾谷镰刀菌的IC50为1.65 mg/mL,对其分生孢子的IC50为2.93 mg/mL,改变禾谷镰刀菌菌丝尖端几丁质的量和线粒体内膜负电性.[结论]提取物具有较好的抑菌活性.
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