[目的]研究农药对农田环境及非靶标有益生物的影响.[方法]选取青海弧菌Q67和斑马鱼作为受试生物,研究多菌灵杀菌剂对其的急性毒性,从而初步评价该杀菌剂农业生产应用中对水生生物的潜在风险.[结果]22%多菌灵杀菌剂对青海弧菌Q67的EC50为7.70 mg/L,pEC50为2.11;22%多菌灵杀菌剂对斑马鱼的24、48、72、96 h LC50分别为8.53、8.39、8.07和7.64 mg/L;斑马鱼的安全浓度为0.76 mg/L.[结论]根据《化学农药环境安全评价试验准则》"鱼类急性毒性试验"对鱼类毒性评价标准,判断22%多菌灵杀菌剂对斑马鱼的毒性属中毒.%[Objective]Effects of carbendazim on agriculture environment and non-target beneficial organisms were studied.[Method]Q67 and zebra fish were selected as a test organism to study the acute toxicity of carbendazim , thus the potential ecological risks of carbendazim to aquatic organisms was preliminarily evaluated .[Result] EC50 to Q67 of 22% carbendazim was 7.70mg/L, pEC50 was 2.11;the 24 h,48 h, 72 h and 96 h LC50 of 22%carbendazim to the testing fish were 8.53,8.39,8.07 and 7.64 mg/L, respectively.The safe concentration of car-benzim to zebra fish was 0.76 mg/L.[Conclusion]Acute toxicity of 22%carbendazim to fish is medium according to the"Fish Acute Toxicity Test" of the Chemical Pesticide Environmental Safety Evaluation Test Guidelines.
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