[Objective] To study the genetic variation of Hippocampus kuda base on D-loop and Cyt b sequences.[ Method] In this paper, the genetic variations of H.kuda between Dongshan island(20 individuals) and Quanzhou City(20 individuals) were analyzed base on part of D-loop and Cyt b sequences.[ Result] D-loop and Cyt b sequences were 707 -709 bp and 404 bp, respectively, and the two sequences were highly conserved between the two populations.G+C content of D-loop and Cyt b sequences were significantly lower than A+T, however, the C content was lower than G, and G content of Cyt b sequences were the least in the third codon.The average number of nucleotide differences of 20 D-loop sequences of H.kuda from Dongshan island and Quanzhou City were 0.489 and 0.958, and 3 and 6 kinds of haplotypes were defined respectively, meanwhile, the similarity of Cyt b sequences were 100％ with a total of 1 haplotype between the two groups.[ Conclusion] All the results showed that the variation of D-loop and Cyt b sequences were relatively little in the two population of H.kuda.%[目的]对大海马D-loop和Cyt b序列进行遗传变异分析.[方法]基于D-loop和Cyt b部分序列对东山岛(20尾)和泉州市(20尾)养殖场大海马进行遗传变异分析.[结果]获得的D-loop和Cyt b序列分别为707～709 bp和404 bp,且2种序列在2个大海马群体之间极其保守.D-loop和Cyt b序列G+C含量明显低于A+T含量,C含量也低于G含量,Cyt b序列第3位密码子G含量最少.东山岛和泉州市的大海马(各20条)D-loop序列的平均核苷酸差异数分别为0.489和0.958,且分别定义了3种和6种单倍型,东山岛和泉州市的大海马(各20条)Cyt b序列在2个群体之间相似度为100％,只定义了1种单倍型.[结论]研究表明D-loop和Cyt b序列在2个大海马群体中变异较小.
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