[Objective]To evaluate the disinfection procedures,choice of disinfectant,feasibility and practical effect of the disinfection method by monitoring the pollution of Salmonella and E.coli in the atmosphere of hatchery room before and after disinfection in a large chicken hatchery of Anhui Province.[Method]The experiment was carried out by natural sedimentation on exposed flat method,with MacConkey culture media as a selective sampling medium.SS medium was used to further identify Salmonella ,and it was confirmed by gram staining and microscopic examination.The number of bacteria in air and health chicks rate in incubator were compared among three different disinfectants and unsterilization.[Result] Salmonella and E.coli were found in each function region of hatchery room after unsterilization for 2 days.With the extension of unsterilization time,the quantity showed an increasing trend.The number of Salmonella and E.coli in the atmosphere of hatchery room reduced to zero after any of three kinds of disinfectants (Anlixiao,peracetic acid,povidone iodine) were used after 2 days.The rate of healthy chicks were significantly improved.[Conclusion] Three kinds of disinfectants (Anlixiao,peracetic acid,povidone iodine)could effectively kill Salmonella and E.coli in the atmosphere of hatchery room with the appropriate diluted concentration,sterilization method and disinfection procedures,so it was suggested that the three disinfectants should be alternately used.%[目的]通过对安徽某大型种鸡孵化场在消毒前后孵化室空气中沙门氏菌和大肠杆菌污染情况的监测,评估该孵化场的消毒程序、消毒剂选择和使用方法的可行性和实际效果.[方法]采用平板暴露自然沉降法,选用麦康凯培养基为选择性采样培养基,SS培养基为沙门氏菌的进一步鉴别培养基,对分离菌进行革兰氏染色、镜检,并对3种不同消毒剂消毒和未消毒时孵化室空气中的细菌数及孵化出雏时的健雏率进行比较.[结果]连续2d未消毒,孵化室各功能区即可检测到沙门氏菌和大肠杆菌,且数量随未消毒时间的延长而增多;分别使用安立消、过氧乙酸、聚维酮碘3种消毒剂任一种连续消毒2 d后,孵化室空气中的沙门氏菌和大肠杆菌数量均降为0,健雏率较未消毒时明显提高.[结论]安立消、过氧乙酸、聚维酮碘3种消毒剂在选择正确的稀释浓度、消毒方法和消毒程序后,可以有效杀灭孵化室空气中的沙门氏菌和大肠杆菌,建议作为孵化室消毒的首选消毒剂交替选用.
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