Objective] To research the rapid propagation technology of Eucalyptus grandis ×E.urophylla DH32 -26 ,and to provide references for the large -scale production.[Method] Half lignification ger mination stem section of Eucalyptus grandis ×E.urophylla DH32 -26 was used as the research material.By using the means of tissue culture,we carried out explant induction and sterile tissue culture system in order.[Result] The optimal explant induction medium was improved MS +6 -BA 1.0 mg /L +NAA 0.5 mg /L,with the ger mination rate being more than 92.3%. The optimal proliferation medium was improved MS +6 -BA 0.4 mg /L +NAA 0.2 mg /L,with number of bud proliferates was 3.7.The best me-dium that the root induce was improved 1 /2 MS +IBA 0.2 mg /L +ABT 0.3 mg /L,the rooting rate was 98.3%.[Conclusion] This research obtains the optimal culture mediums and cultivation conditions of initial induction,proliferation and rooting of Eucalyptus grandis ×E.urophylla DH32 -26 ,so as to preli minarily meet the requirements of industrialized production.%[目的]探索尾巨桉 DH32－26组培快繁技术，为其规模化生产提供参考。[方法]采用组织培养手段，以尾巨桉 DH32－26半木质化萌发茎段为材料，对外植体诱导及无菌组织培养体系进行研究。[结果]最佳外植体诱导培养基为改良 MS ＋6－BA 1．0 mg ／L ＋NAA 0．5 mg ／L，发芽率为92．3％；最佳增殖培养基为改良 MS ＋6－BA 0．4 mg ／L ＋NAA 0．2 mg ／L，增殖个数为3．7；最佳生根培养基为改良1／2MS＋IBA 0．2 mg ／L ＋ABT 0．3 mg ／L，生根率可达98．3％。[结论]该研究获得了尾巨桉初代诱导、增殖和生根适宜培养基和培养条件，初步达到工厂化生产要求。
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