[目的]探讨云南小粒速溶咖啡中百菌清的最佳提取净化方法。[方法]分别采用石油醚－丙酮萃取法、固相吸附法及二氯甲烷－丙酮萃取法这3种不同的方法提取净化云南小粒速溶咖啡中的百菌清，并利用高效液相色谱进行加标回收测定，根据回收率的大小和净化方式的繁简，确定最适宜的百菌清测定方法。[结果]3种不同方法提取净化百菌清的加标回收率分别为85．3％、111．4％和118．7％。[结论]通过回收率及净化方式的比较可知，可利用固相吸附法对云南小粒速溶咖啡中的百菌清进行提取纯化。%Objective] The aim was to explore the optimal extraction and purijication method for chlorothalonil in Yunnan small grain instant coffee.[ Method] Three different extraction and purification methods ( aether petrolei-acetone extraction, solid-phase adsorption and dichlo-romethane-acetone extraction) were performed to extract chlorothalonil in instant coffee .Then all of the extractions were tested using high per-formance liquid chromatography(HPLC).The most suitable method was determined according to the percentages of recovery measurement. [Result] The results obtained by three different extraction and purification methods showed that the recovery percentages were 85.3%, 111. 4% and 118.7%, respectively.[Conclusions] By comparing recovery rate and purification methods, solid-phase adsorption method was the best one for testing chlorothalonil in Yunnan small grain instant coffee.
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