[目的]对中香梨的离体培养进行研究.[方法]以中香梨茎尖和嫩叶为外植体,筛选适合中香梨茎尖培养及叶片愈伤组织诱导的最佳培养基.[结果]中香梨茎尖培养的最佳培养基为MS+ 1.5 mg/L 6-BA +0.15 mg/L NAA +30 g/L蔗糖+6 g/L琼脂；叶片的最佳愈伤组织诱导培养基为MS+ 1.0 mg/L 6-BA+1.0 mg/L IBA+ 40 g/L蔗糖+6 g/L琼脂.[结论]通过茎尖培养可很快获得中香梨植株,但从愈伤组织诱导植株较为困难.%[Objective]The aim was to study the in vitro culture of Pyrus bretschneideri Rehd. rnrncv. ' zhongxiang'. [ Method ] The optimalrnmedia of shoot tip culture and leaf callus induction of P. bretschneideri rnrnRehd. cv. ' zhongxiang' were screened by using shoot tips and tenderrnleaves as explants. [ Result] The optimal shoot tip culture medium of P. rnrnbretschneideri Rehd. cv. ' zhongxiang' was MS +1.5 mg/L 6-BA +rn0. 15 mg/L NAA +30 g/L Sugar + 6 g/L Agar; the best callus induction rnrnmedium of leaf was MS + 1.0 mg/L 6-BA + 1.0 mg/L IBA +40 g/LrnSugar+ 6 g/L Agar. [Conclusion] It would be quickly to obtain P. rnrnbretschneideri Rehd. cv. 'zhongxiang' plantlet by shoot tip culture, butrndifficult to induce plant from callus.
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