[目的]建立用静态电化学发光分析法检测环孢霉素的方法.[方法]以实验室自合成的羧基钌为发光试剂,环境友好型二丁基乙醇胺为共反应试剂,用静态电化学发光分析法检测环孢霉素.[结果]在pH-为6.0的0.06 mol/L PBS缓冲溶液中,羧基钌发光强度的淬灭值与环孢霉素A浓度在9.3×10-9 ～4.0×10-7 mol/L之间呈良好的线性关系(R2=0.9950),检测限达5.4×10-7 mol/L.[结论]该方法是首次用电化学发光方法应用于环孢霉素的检测,为电化学发光方法检测环孢霉素提供了理论与试验依据.%[ Objective ] A static electrochemiluminescent approach was developed to determine cyclosporine A. [ Method ] By using the synthetic carboxyl ruthenium as luminescent reagents and environmental friendly dibutyl ethanolamine as the co-reaction reagent, cyclosporine was determined by static electrochemiluminescence analysis. [Result] Linear responses in chemiluminescence quenching of carboxyl ruthenium and concentration of cyclosporine A were good linearity ( R2 = 0.995 0 ) between 9.3×10-9 mol/L and 4.0 × 10-7 mol/L in 0.06 mol/L PBS buffer solution (pH 6.0). The corresponding limit of detection (LOD) was 5.4 × 10-7 mol/L. [Conclusion] The method is suitable for the determination of cyclosporine by electrochemiluminescence for the first time and provides theoretical and experimental supports.
展开▼
