[目的]建立高效液相色谱法测定指甲油中苏丹红Ⅱ、苏丹红Ⅳ含量的方法.[方法]采用HPLC法对指甲油中苏丹红Ⅱ、苏丹红Ⅳ进行含量测定.色谱条件为:C18柱(3.5 μm,4.6mm×150 mm),柱温30℃,流动相为乙腈-缓冲溶液梯度洗脱,流速1.0 ml/min,检测波长484nm及514 nm切换.[结果]苏丹红Ⅱ、苏丹红Ⅳ色谱分离良好,浓度与峰面积呈良好线性关系,线性范围分别为苏丹红Ⅱ0～50.26 μg/ml,r =0.999 8(n =6)；苏丹红Ⅳ0～49.78μg/ml,r=0.999 9(n =6).平均加样回收率分别为95.9％(n=9,RSD=2.87％)、87.7％(n=9,RSD =0.4％).[结论]该方法测定了5批指甲油中苏丹红Ⅱ、苏丹红Ⅳ的含量,分离度好、快速、简便、重现性好.%[Objective] To establish an HPLC method for determination of Sudan Ⅱ and SudanⅣ in nail polish.[Method] HPLC analysis was performed on C18 column (4.6 mm × 150 mm,3.5 μm) with the acetonitrile-buffer solution as mobile phase in gradientmode,the column temperature was setup 30 ℃,the flow rate was 1.0 ml/min and the detecting wavelength was at 484 mn and 514 nm switching.[Result] The linear response ranges from 0 to 50.26 μg/ml of Sudan Ⅱ (r =0.999 8,n =6) and from 0 to 49.78 μg/ml of Sudan Ⅳ (r =0.999 9,n =6) ;the recoveries of quercetin,apigenin and luteolin were 95.9％ (n =9,RSD =2.87 ％),87.7 ％ (n =9,RSD =0.40％),respectively.[Conclusion] All 5 samples were determined by HPLC.The method is a practicable method in determination of Sudan Ⅱ and SudanⅣ in nail polish.
展开▼
