[目的]克隆马立克氏病鸡MDV血清基因,并对其进行鉴定.[方法]从潜伏期感染马立克氏病鸡淋巴组织中提取基因组DNA,采用梯度PCR扩增马立克氏病毒的L-meq基因.将其插入pMD18-T克隆载体并进行测序,并用DNAman软件进行序列分析.[结果]获得的DNA片段的序列与已发表的L-meq基因序列相一致,表明该试验成功获得了目的基因.[结论]该研究可为鸡马立克氏病的预防与治疗提供新思路和新方法.%[Objective] To clone and identify Marek' s disease virus (MDV) serum gene. [ Method] MDV genomic DNA was extracted from lymphoid tissues of the diseased chickens with latent MDV infection. The MDV L-meq gene was amplified by gradient PCR,inserted into pMD18 - T vector and sequenced. The sequence was analyzed using DNAman software. [ Result ] The obtained sequence had 100% similarity with the published sequence of L-meq gene,showing successful amplification of target gene. [Conclusion] The paper provides new ideas and new methods for prevention and treatment of Marek' s disease in chickens.
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