[目的]研究附子总生物碱的提取工艺,优选最佳提取条件.[方法]以提取率、总生物碱收率和总生物碱纯度为指标,采用单因素试验法,考察提取溶剂、提取方法、提取温度、粉碎度、料液比、提取时间和提取次数对附子中总生物碱提取率的影响,并优选出最佳提取工艺条件.[结果]最佳提取工艺条件为:在15℃条件下,将附子粉按1∶10(g/ml)加入无水乙醇,冷浸3次,每次1d；在此条件下,浸膏得率、总生物碱收率和总生物碱纯度分别为3.15％、0.64％和20.32％.[结论]该方法高效、低毒、经济、操作方便、工艺可控,较适合附子总生物碱的提取和工业化生产.%[ Objective ] To determine the optimal extraction technology of alkaloids from the roots of RADIX ACONITI LATERALIS PRA-EPARATA. [ Method ] Taking extraction rate, and the purity and yield of total alkaloid as the indices, effects of extraction solvent, extraction method, extraction temperature, extraction time ,comminution degree, solid-liquid ratio and extraction times on the extraction rate of total alka-loids were studied. And the optimal extraction technology was selected. [ Conclusion ] The optimal extraction technology was as follows : RADIXACONITI LATERALIS PRAEPARATA powder was added into anhydrous alcohol with the ratio of 1:10 (g/mt)under 15 ℃. After cold leaching for 3 times with each time for 1 d, the dry extract rate, extraction rate of total alkaloid, and purity of total alkaloid were 3.15% ,0.64% and 20. 32% ,respectively. [ Conclusion] This method was efficient,low toxic, economical,convenient and can be used for industrial production.
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