[Objective] To study the content change of polysaccharides in 3 Huoshan species of Dendrobiumn with the variation of time. [ Method ] The content of polysaccharides in 3 Huoshan species of Dendrobiumn including Huoshan D. Officinale, D. Huoshanense and D. Monil-iforme at 8 growth stages was determined by sulfuric acid-phenol method. And, the changing trend and impacting factors were also analyzed. [ Result] The content of polysaccharides was increasing steadily based on a low level at the stage of tissue culture, and it decreased in the following order, D. Officinale, D. Huoshanense, D. Moniliforme. The highest content of polysaccharides in those Dendrobium was pursued after two years culture, the order was D. Huoshanense > D. Qfficinale > D. Moniliforme, among which the content of polysaccharides in D. Huoshanense was as high as 35.74% . [ Conclusion] Those results lay a foundation for the breeding, cultivation and application of those three kinds of Dendrobium.%[目的]研究霍山产3种石斛多糖含量变化规律.[方法]采用硫酸-苯酚法测定霍山石斛、霍山铁皮石斛和霍山细茎石斛3种霍山产石斛在8个不同生长阶段的多糖含量,并分析了其变化的规律和影响因素.[结果]在组培苗繁育阶段,石斛多糖含量在相对较低的水平上稳步增长,且呈现出霍山铁皮石斛＞霍山石斛＞霍山细茎石斛的规律；石斛在栽培2年后多糖含量达到最高,品种间的规律表现为霍山石斛＞霍山铁皮石斛＞细茎石斛,其中霍山石斛2年生茎多糖含量高达35.74％,在3种石斛中是最高的.[结论]试验研究了霍山产3种石斛多糖含量变化规律,为石斛的繁育、栽培以及应用提供了依据.
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