[目的]探讨林蛙皮活性肽的抗氧化活性.[方法]林蛙皮经木瓜蛋白酶水解后,将产物经葡聚糖凝胶G-25层析纯化,测定活性组分对DPPH·、·OH、O2-·的清除能力.[结果]柱层析共分离出4个组分(PⅠ、PⅡ、PⅢ、PⅣ),各组分抗氧化能力的大小顺序依次为PⅡ＞PⅢ＞ PⅠ＞ PⅣ.PⅡ组分对各自由基清除能力的大小顺序依次为DPPH·＞ · OH＞O2-·.蛋白浓度为1mg/ml时,对DPPH·的清除率为94.23％；蛋白浓度为16 mg/ml时,对OH的清除率为86.79％,对O2-·的清除率仅为26.32％.[结论]该研究为林蛙的综合开发和利用提供了新的依据.%[Objective] The aim was to study the aminxidant activity of active peptide from skin of forest frog. [Method] The papain hydrolysate of skin of forest frog was purified by Sephadex G-25 gel filtration,and the scavenging activity of DPPH??稯H and O2-?of active components was determined. [Result] The results showed that there were 4 components were obtained,the antioxidant activities were in the order:P? > P? >P?> P?. The order of radical scavenging activity of fraction P? was DPPH?>稯H > O-2?The scavenging rate of DPPH- was 94.23% when the concentration of the protein wag 1 mg/ml ,and the scavenging rate of 稯H and O2- ?were 86.79% and 26.32% respectively when the concentration of the protein was 16 mg/ml. [ Conclusion ] The study provides a new basis for the comprehensive development and utilization of forest frog.
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