[目的]优选AB-8型大孔树脂精制天麻钧藤饮药液的工艺务件.[方法]以天麻素的含量和出膏率为考察指标,采用单因素和L9(36)正交法对树脂纯化工艺中的最大上样量、上样速度和洗脱剂浓度等参数进行优选.[结果]上柱前平均出膏率为21.252 7％,RSD为1.0096％;天麻素的平均含量为5.263mg/g,RSD为0.693％.最佳工艺为最大上样量30ml、上样速度2 BV/h、洗脱溶剂为浓度40％乙醇、洗脱剂用量165 ml、药液浓度0.5 g/ml、径高比1:10;在此条件下,浸膏得率为9.98％,天麻素平均含量为13.364 mg/g.[结论]该方法合理可行,提高了天麻素的含量,并降低了浸膏量.%[Objective] To optimize the refined Tianma Gouteng decoction by AB-8 macroporous resin. [Method] With gastrodin content and paste-forming rate as the indices, the maximum sample volume. Loading rate, eluent concentration and other factors were optimized during the purification process by single factor test and orthogonal test. [ Result] The average paste-forming rate was 21.252 7% before resin purification process and RSD was 1.009 6% ; the average gastrodin content was 5.263 mg/g and RSD was 0.693%. The optimal process was as follows: 30 ml maximum sample volume, 2 BV/h loading rate, 40% ethanol as elution solvent, 165 ml elution volume, 0.5 g/ml liquid concentration, and 1 :10 diameter-height ratio. Under this condition, the dry extract rate was 9.98% and the average content of gastrodin was 13.364 mg/g. [Conclusion] This method was reasonable and feasible, could enhance the content of gastrodin, and reduce the extracts amount.
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