[目的]优化大孔吸附树脂法纯化荔枝壳总黄酮的工艺.[方法]比较AB-8、HPD-600和D101 3种大孔吸附树脂对荔枝壳总黄酮的吸附和解吸效果,并对上柱液的pH、黄酮浓度、上柱液体积和洗脱液乙醇体积分数等条件进行优化.[结果]D101大孔吸附树脂适宜荔枝壳总黄酮的提纯,其最佳工艺条件为上柱液pH 5.0,上柱液浓度4 mg/ml,上柱液体积2.5 BV,洗脱液乙醇体积分数80％,洗脱体积2.0 BV.[结论]经D101大孔吸附树脂分离后,荔枝壳总黄酮含量在83％以上.%[ Objective ] This study aimed to optimize the conditions for purification of total flavones from litchi pericarp by macroporous absorption resin. [Method] The flavones adsorption rate and desorption rate of macroporous absorption resins (AB-8, HPD-600, D101) were compared,and the technological parameters of D101 during the purification process were investigated,including on-column liquid pH,flavone-concentration,on-column liquid volume and volume fraction of elution solution. [Result] D101 macroporous absorption resin is appropriate for the purification of total flavonoids from litchi pericarp. The optimal technological conditions were;on-column liquid pH =5.0; on-column liquid concentration was 4 mg/ml,with a volume of 2.5 BV; elution solution was 80% ethanol,with a volume of 2.0 BV. [Conclusion] The content of total flavones achieve 83% by using the method.
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