[ Objective] To study the in vitro rapid propagation system of D. nobile. f Method] The seeds of D. nobile were selected for the inrnvitro rapid propagation by tissue culture. [ Result] MS + I mg/L BA + 0.02 mg/L N AA + 2% potato juice + 3 % sucrose was suitable forrnthe protocorm induction and hud differentiation of D. nobile. The inductive rate was 7 - 10. The most suitable rooting medium was 1/2 MS +rn0. 2 mg/L BA + 0.2 mg/L NAA + 0.2 mg/L GA + 2% banana juice + 2% potato juice + 2% sucrose. The rooting rate was above 98% ,rnand survival rate was above 90%. [ Conclusion ] The method studied the in vitro rapid propagation system of D. nobile and provided referencesrnfor the tissue culture of its seedlings.%[目的]研究金钗石斛的离体快繁体系.[方法]以金钗石斛的种子为材料,采用组培法进行离体培养.[结果]适宜于金钗石斛圆球茎诱导增殖及茅分化的培养基为:MS+1 mg/L BA +0.02 mg/L NAA+浓度2％马铃薯+浓度3％白糖,增殖系数为7-10.生根培养基为1/2MS +0.2 mg/L BA +0.2 mg/L NAA +0.2 mg/L CA+浓度2％香蕉+浓度2％马铃薯+浓度2％白糖;生根率达98％以上,移栽成活率在90％左右.[结论]该方法对金钗石斛的离体快繁体系进行了研究,为组培繁殖金钗石斛种苗提供了参考.
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