[Objective] To optimize the supercritical CO2 process parameters of essential oils from PINE NEEDLES and to identify the chemical constituents of essential oils. [ Method ] Taking the yield of essential oils as an index, the proper factors and levels were chosen through the pre-test, and the interaction between factors was observed by orthogonal test. Moreover, GC-MS analysis was used to identiy the chemical constituents of essential oils. [ Result] The optimized technology of supercritical CO2 extraction of essential oils from PINE NEEDLES were extraction pressure 18 Mpa, extraction temperature 40 ℃ , CO2 flow 25 iyh, and separation 1 pressure 8 Mpa. The yield of essential oils was as much as 0.163% using the optimized process parameters. The main chemical constituents of essential oils were a-pinene, p-pinene, and carypohyllene with content more than 50%. [ Conclusion] This method can screen out the supercritical CO2 process parameters of essential oils from PINE NEEDLES. It can provide basis for development and utilization of essential oils from PINE NEEDLES.%[目的]优选超临界CO2萃取马尾松针精油的工艺参数,并对其成分进行测定.[方法]以松针精油得率为指标,通过预试验选择了合适的因素和水平,进一步通过正交试验观察了各因素之间的相互作用,并优选出最佳的提取工艺条件；然后采用GC-MS对其所含化学成分进行研究.[结果]优化得到的超临界CO2萃取马尾松针精油的最佳工艺为:萃取釜压力18 MPa,萃取温度40℃,CO2流量25L/h,分离Ⅰ压力8 Mpa;在此优化工艺参数条件下,松针精油的得率高达0.163％；成分分析结果表明,其精油主要成分为α-蒎烯、β-蒎烯和石竹烯,且含量在50％以上.[结论]该方法优选出了马尾松针精油的最佳超临界CO2提取工艺参数,为马尾松针精油的开发利用提供了依据.
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