[Objective] The aim was to find a way to measure the cholesterol content in deserted oil. [Method] Dual-wavelength spectropho-tometry method was used for the determination of cholesterol in edible oil. 630 nm and 720 nm were set as target band and reference band in this method, respectively. The result was compared to liquid chromatography method. [Result] The relative standard deviaiion(RSD)was at 1.26% and recovery was between 101.9% and 110.7%. Linear range was 8.0 -40.0 礸/ml. The tested result was close to the result of liquid chromatography. [Conclusion] It was proved that this method was easy, fast and accurate. The disturbance of sitosterol was eliminated without isolation. It can be used for fast identification of the vegetable oil containing waste fat%[目的]建立废弃油脂中胆固醇含量的测定方法.[方法]采用双波长分光光度法测定食用油中胆固醇含量,以630 nm为测定波长,720nm为参比波长进行测定,将测定结果与液相色谱法结果进行比较.[结果]该方法精密度RSD为1.26％,加标回收率101.9％ ～110.7％,线性范围8.0～40.0μg/ml,试验结果与液相色谱法测定结果接近.[结论]经实际样品验证,双波长分光光度法具有操作简便、快速、准确度好等特点,且无需分离即可消除谷甾醇的干扰,可用于含废弃动物油脂的植物油的快速鉴定.
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