[目的]研究中国水仙生物碱的提取工艺,以期为水仙生物碱的提取提供指导方法.[方法]运用正变法对提取时间、提取温度、料液比3个因素进行优化,并对萃取溶剂和萃取次数进行筛选.[结果]水仙生物碱提取的最佳试验条件为:料液比1∶20,提取温度70℃水浴,提取时间4h,萃取溶剂选用三氯甲烷,萃取2次.在该最优条件下提取生物碱的得率达到0.077％.[结论]该研究结果表明水仙是提取生物碱的良好材料,这对于水仙的经济价值的开发具有积极的意义,同时也将促进生物碱的开发利用.%[Objective] To study the extraction technology of alkaloids from Narcissus tazetta var. chinensis Roem, so as to supply a suitable extraction method. [Method] The orthogonal experiment was used to optimize the extraction time, extraction temperature and solid-liquid rati-o, and screen out the solvents and extraction times. [Result] The best extraction conditions of alkaloids were 1:20 solid-liquid ratio, 70 ℃ of extraction temperature and 4 h extraction time, using chloroform as extraction solvent for twice. Under the optimized conditions, the extraction rate of alkaloids reached up to 0.077% . [Conclusion] Narcissus tazetta var. chinensis Roem is the ideal source for alkaloids extraction, which has positive significance to developing the economic value of Narcissus tazetta var. chinensis Roem and promoting the exploitation of alkaloids.
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