[目的]对悬铃木的组织培养进行研究,为实现其优良品系的大量繁殖和保存提供指导方法.[方法]以4个优良家系的悬铃木单株为试验材料,探讨其在不同培养基上的增殖情况,筛选出各自适合的增殖培养基.[结果]在芽增殖上,4个无性系间、培养基间及无性系与培养基间的交互作用均存在极显著差异.通过对增殖培养的方差分析及多重比较,筛选出了SX4、SX12、SJ28和DY184个无性系的最适增殖培养基分别为A4、A2、A2和A4,其中以SX4的增殖效果最好.[结论]该研究结果为悬铃木的组培提供了指导方法,为其进一步深入研究提供了材料基础.%[Objective] The aim was to carry out study on the tissue culture of Platanus occidentalis L. so as to provide suitable method for propagation and preservation of fine strains. [Method] Four lines of P. occidentalis were used as test materials to explore the proliferation conditions of them on different media. [Result] The difference on the proliferation culture conditions among four clones, four media as well as the interaction of clones and mediums is extremely significant. The multiple comparison result finds that the optimal proliferation media for SX4, SX12, SJ28 and DY18 are A4, A2, A2 and A4. SX4 shows the best proliferation result. [Conclusion] The result in this study has provided suitable method for propagation and preservation of fine strains and the material basis for further studies on P. occidentalis.
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