[目的]对沈氏栎组培快繁技术进行初步研究,以期为该物种的快速繁殖和优良性状的保持奠定基础.[方法]以引自北美的沈氏栎(Quercus shumardi’Shumard Oak)为试验材料,从外植体接种试验、试管苗的增殖与生根等技术环节进行了探索,研究沈氏栎瓶内培养技术体系.[结果]用作外植体的理想材料为带腋芽茎段；最佳灭菌方式是用75％的酒精消毒1 min后再用0.1％升汞消毒6 min；试管苗增殖(诱导丛生芽)的最佳培养基为MS +6-BA 0.80 mg/L+NAA 0.45 mg/L;诱导生根的最佳培养基组合为1/2 MS+ NAA 0.40mg/L；瓶苗培养的理想温度为26 ～ 28 ℃.[结论]该研究初步建立了沈氏栎瓶内培养技术体系,同时为该物种的快速繁殖和优良性状的保持奠定基础.%[Objective] To study the rapid tissue culture of Quercus shumardi' shumard Oak',so as to lay foundation for the rapid propagation of this species and maintaining its excellent traits. [ Method] The Quercus shumardi' shumard Oak' from North America was collected as material,the tissue culture and rapid propagation technology was studied from explants inoculation,test-tube proliferation and rooting and so on. [Result] The ideal material of explants was stems with auxiliary bud; the disinfection efficiency of explants was better with 0. 1% HgCl2 for 6 min after the treatment with 75% alcohol for 1 min; the optimum medium for auxiliary buds induction was MS +0. 80 mg/L 6-BA +0.45mg/L NAA; 1/2MS + 0.40 mg/L NAA was suitable for the test tube plantlet rooting; Test-tube seedling raised ideal temperature for 26 -28 ℃. [ Conclusion] The study established a bottle culture system for Quercus shumardi' shumard Oak' and laid foundation for its rapid propagation and maintaining its excellent traits.
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