[Objective] The research aimed to establish a standard Fourier transform infrared (FT-IR) spectral library and a FT-IR method of differentiation and identification of Staphylococcus aureus and Staphylococcus epidermidis. [Method] FT-IR fingerprint absorption spectra of two species of Staphylococcus were collected and analyzed by chemometric methods- [ Result ] A standard FT-IR spectral library was created and could be used to compare with those of the target Staphylococcus and identify them. A cluster model of principal component analysis was established and could differentiate and identify well the two species of Staphylococcus. [Conclusion] As a rapid, easy-to-use and accurate technique , FT-IR spectroscopy is an effective tool to differentiate and identify two species of Staphylococcus.%[目的]建立金黄色葡萄球菌和表皮葡萄球菌2种菌的傅立叶变换红外光谱数据库及FT-IT分类鉴定方法.[方法]应用FT-IR技术对2种葡萄球菌进行指纹图谱数据采集,应用化学计量学分析方法对光谱进行分析.[结果]建立2种葡萄球菌的标准FT-IR光谱数据库,用于FT-IR技术对2种可疑目标葡萄球菌进行鉴定；建立一种基于主成分分析的聚类分析模型,可成功地区分2种葡萄球菌.[结论]傅立叶变换红外光谱分析方法简便、快速、易操作,结果重现性好,是一种区分金黄色葡萄球菌和表皮葡萄球菌的有效方法.
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