[目的]为进一步提高海洋菌Bacillus sphaericus M4产多糖的量,对其发酵产多糖的条件进行优化.[方法]分别研究发酵温度、转速对发酵的影响,并通过两段法对Bacillus sphaericus M4产多糖的条件进行优化.[结果]菌体最适生长温度为31℃,最佳转速为200r/min；两段法优化的结果表明:摇瓶转速由200 r/min降为100 r/min,多糖产量会由8.32 g/L提高至10.55 g/L;温度由31℃升至34℃C,多糖产量会由10.35 g/L提高至12.16 g/L.[结论]为Bacillus sphaericus M4胞外多糖的工业化生产奠定了基础.%[ Objective ] To further improve the yield of polysaccharide, the fermentation conditions of marine bacteria Bacillus sphaericus M4 were optimized. [ Method ] The effects of fermentation temperature and rotation speed on fermentation were studied, and the polysaccharide production conditions of Bacillus sphaericus M4 were optimized by two-stage method. [ Result] The optimum growth temperature of bacteria was 31℃, the optimum rotation speed was 200 r/min. The result of two-stage optimization method showed; when the rotation speed of shake flask was decreased from 200 r/min to 100 r/min, the yield of polysaccharide was increased from 8. 32 g/L to 10. 55 g/L; when the temperature was increased from 31℃ to 34℃ , the yield of polysaccharide was also increased from 10. 35 g/L to 12.16 g/L. [ Conclusion] It prepared the ground for the industrialized production of exopolysaccharide of Bacillus sphaericus M4.
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