[目的]对中药材大黄的有效期进行研究.[方法]选用易氧化变色的大黄,通过经典恒温试验,采用HPLC测定大黄酸、大黄素、大黄素甲醚、芦荟大黄素的含量,并研究该药有效成分含量的变化规律.[结果]经典恒温法测定的大黄酸、大黄素、大黄素甲醚、芦荟大黄素含量变化符合一级反应规律.在20℃的储藏条件下,大黄有效成分的含量变化速度常数为K=1.585×10-5,该批大黄有效期为687 d.[结论]用经典恒温法预测中药材大黄有效期,结论可靠.%[Objective] To study the period of validity of traditional Chinese medicine rhubarb. [Method] Easily oxidized rhubarb was selected. Based on classic constant temperature experiment, contents of rhein, archen, physcion and aloe-emodin were detected by HPLC. The change law of the effective components contents in rhubarb was deduced. [Result] Changes of rhein, archen, physcion and aloe-emodin contents were followed the first-order reaction law. Under the storage condition of 20℃ , the change rate constant of rhubarb was K = 1. 585 × 10-5; and the period of validity of this batch of rhubarb was 687 d. [ Conclusion] It was feasible to forecast the validity period of rhubarb by classic constant temperature experiment, and the result was reliable.
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