[ Objective ] To extract high quality total RNA from the bark of Eucommia ulmoides Oliv and lay foundation for the isolation of mRNA,cloning of anti-fungi protein gene and library construction of cDNA. [ Method ] Total RNA from the bark of Eucommia ulmoides Oliv was extracted by modified CTAB-LiCl method, RNApure Plant Kit method and RNAiso Plus method respectively. The quality of total RNA was examined through ultraviolet spectrophotometer and formaldehyde denaturalization agarose gel electrophoresis. [ Result ] The results showed that total RNA extracted by modified CTAB-LiCl method and RNApure Plant Kit method had higher purity,integrity and yield rate,the ratio of A260/A260 was in the range of 1.8 ～2.0,the bands of 28S and 18SrRNA were clear; while the quality of total RNA obtained by RNAiso Plus method was poor,the ratio of A260/A260 was 1. 652 ,and the band of RNA was diffusion,which suggested the total RNA was degraded during the extraction process. [ Conclusion]The results showed that total RNA from the bark of Eucommia ulmoides Oliv extracted by modified CTAB-LiCl method and RNApure Plant Kit method was of high purity and quality,which could meet the requirement of RT-PCR and RACE etc. experiments as well as lay the foundation for cloning genes from Eucommia ulmoides Oliv .%[目的]从杜仲树皮中获得高质量的总RNA.为开展杜仲后续mRNA分离、杜仲抗真菌蛋白基因克隆、及杜仲树皮cDNA文库构建等研究奠定基础.[方法]以杜仲树皮为材料,分别采用改良CTAB-LiCl法、RNApure Plant Kit法和RNAiso Plus法进行总RNA提取,用琼脂糖甲醛变性凝胶电泳检测总RNA的完整性,用紫外分光光度计检测总RNA的纯度和得率.[结果]改良CTAB-LiCl法和RNA-pure Plant Kit法提取的杜仲树皮总RNA纯度和完整性较高,A/A值均在1.800～2.000,28和18 S rRNA条带清晰,RNA得率较高;而RNAiso Plus法提取的杜仲树皮总RNA的纯度和完整性较低,A/A值为1.652,28S和18S rRNA条带存在降解和弥散现象,RNA得率低.[结论]改良CTAB-LiCl法和BNApure Plant Kit法抽提获得的杜仲树皮总RNA质量较高,能满足后续RT-PCR和RACE等试验的要求,这为成功克隆杜仲相关基因奠定了基础.
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