[ Objective ] The best testing condition of four organic acids( tartaric acid, citric acid, L-malic acid and succinic acid) in tropical fruit-Eugenia javanica Lam was selected through the research on the influence of the different testing conditions on chromatographic separation of four organic acids. [ Method] The testing condition was optimized based on the orthogonal experiment with four factors at 3 level( including wavelength, buffer, rate of buffer to methanol in volume ratio, flowing rate of mobile phase and the temperature of column. [ Result] In the column(4.6 mn × 250.0 mm ,5 μm) of Zorbax Eclipse XDB C18, the four organic acids in Eugenia javanica Lam could be better tested and isolated while the detection wavelength was 210 nm, 10％ CH3 OH - 0. Ol mol/L NaH2 PO4 ( pH 2.28 was used as the mobile phase, the flowing rate was 0.8 ml/min and the temperature of column was used at 40℃. [ Conclusion]Tbe results mentioned above indicated that the method was simple,rapid and accurate, which was significant in the control of fruit quality.%[目的]研究不同检测条件对热带水果莲雾中4种有机酸(酒石酸、柠檬酸、L-苹果酸、琥珀酸)色谱分离的影响,以确定最佳的试验条件.[方法]通过波长扫描确定检测波长,3种不同pH值的缓冲溶液、缓冲溶液与甲醇的体积比、4种流动相流速及5种条件柱温,按照4因素3水平的正交试验对分离条件进行优化.[结果]在Zorbax Eclipse XDB C(4.6 mm×250.0 mm,5 μm)色谱柱上,当紫外检测波长为210 nm,10%CHOH-0.01 mol/L NaHPO(pH值2.28)混合溶液作流动相,流速为0.8 ml/min,柱温40℃时,可以较好地分离和测定热带水果莲雾中4种常见有机酸.[结论]该方法具有前处理简单、灵敏度高、测试组分多、速度快等优点,对于控制果品质量具有重要的意义.
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