[目的]获得铁皮石斛(Dendrobium candidum Wall.ex Lindl.)原球茎组织培养的最佳培养基配方.[方法]用正交试验筛选出6-BA、NAA、KT 最佳的浓度配比,并考察各因素对铁皮石斛原球茎增殖率的影响.[结果]铁皮石斛原球茎增殖的最佳培养基配方为1/2MS+2.0 ms/L 6-BA+0.5 ms/L NAA+1.0 ms/L KT.[结论]该研究为工业化生产铁皮石斛原球茎及其有效成分,推进铁皮石斛工厂化育苗奠定了基础.%[ Objective ] To obtain the optimum medium formula for protocorm proliferation of Dendrobium candidum Wall. ex Lindl.. [ Method ] The optimal concentration formula of 6-BA, NAA and KT was screened by orthogonal test, and effect of each factor on proliferation rate of protocorm from Dendrobium candidum Wall. ex Lindl. was investigated. [ Result] The optimum medium formula was: 1/2MS + 2.0 mg/L 6-BA + 0.5 mg/L NAA + 1.0 mg/L KT. [ Conclusion] This study provided basis for industrialized production of protocorm proliferation of Dendrobium candidum Wall. ex Lindl. and its active compositions,and for advancing the industrialized breeding of Dendrobium candidum Wall ex Lindl..
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