[Objective] To optimize the supercritical CO2 fluid extraction process for aloe polysaccharide,so as to obtain high-quality aloe polysaccharide. [ Method] The static and dynamic extraction duration were optimized by single factor test ,and the extraction kettle conditions were optimized by orthogonal test. [ Result] The optimum supercritical CO2 fluid extraction conditions were as following: 2.5 ml/g of ethanol amount,extraction pressure of 25 MPa and extraction temperature of 35℃. In addition,the optimal static and dynamic extraction duration were 60 and 30min, individually. Under the optimal conditions, the yield of aloe polysaccharide reached as high as 85.10%. [ Conclusion] Gentle in extraction conditions,the environment-friendly and energy-saving supercritical CO2 fluid extraction technology is favorable for extracting polysaccharide from aloe.%[目的]优化超临界CO 流体萃取芦荟多糖的工艺,以获得高纯度的芦荟多糖.[方法]采用单因素试验对动、静萃取时间进行优化,采用正交试验优化萃取釜条件.[结果]超临界CO 萃取芦荟多糖的最佳工艺为:乙醇用量2.5 ml/g,萃取压力25 MPa,萃取温度35℃.静萃取最佳时间为60 min,动萃取时间为30 min,在最优条件下,芦荟多糖得率为85.10%.[结论]超临界CO 流体萃取条件温和、环保、节能,适用于芦荟多糖的提取.
展开▼